AHK (Tripeptide-3) 200mg

AHK (Tripeptide-3) 200mg | High-Purity Research Grade Peptide

Comprehensive Overview of AHK (Tripeptide-3) 200mg

The AHK (Tripeptide-3) 200mg formulation is a highly purified research compound consisting of the amino acid sequence Alanyl-Histidyl-Lysine. While frequently studied in its copper-bound form, the “Basic” AHK variant is a critical tool for researchers investigating the intrinsic signaling properties of the peptide chain itself. This 200mg presentation is specifically engineered for advanced laboratory settings, providing the necessary volume for extensive in vitro and ex vivo experimental protocols.

In biochemical research, AHK is recognized for its potential role in modulating cellular proliferation and the synthesis of the extracellular matrix (ECM). Researchers utilize this high-concentration 200mg vial to study the peptide’s influence on fibroblasts and its ability to regulate the biological markers associated with tissue growth and structural protein production.

Technical Specifications and Biochemical Profile

Molecular Architecture and Synthesis Purity

The efficacy of AHK (Tripeptide-3) in a laboratory environment is contingent upon its molecular precision. Our 200mg vials are synthesized using state-of-the-art Solid Phase Peptide Synthesis (SPPS) and undergo rigorous purification to ensure experimental reproducibility.

• Amino Acid Sequence: Ala-His-Lys

• Molecular Formula: $C_{15}H_{26}N_{6}O_{4}$

• Molecular Weight: Approximately $354.41 \text{ g/mol}$

• Purity Level: $\ge 99\%$ (Verified via HPLC and MS)

• Form: Sterile, Lyophilized White Powder

Solubility and Reconstitution Protocols

To maintain the bio-activity of the AHK 200mg vial, adherence to standardized laboratory handling is required. The peptide is highly hydrophilic and demonstrates excellent solubility in common research solvents.

1. Solvent Compatibility: Best reconstituted in sterile deionized water or Phosphate Buffered Saline (PBS).

2. Preparation: Introduce the solvent slowly to avoid mechanical shear; a gentle swirling motion is sufficient for complete dissolution.

3. Storage Guidelines: The lyophilized powder is stable at room temperature for transit. For long-term preservation, store at -20°C. Once reconstituted, the solution should be refrigerated at 2°C to 8°C and utilized within a 10-day window to maintain peak stability.

Research Applications and Laboratory Utility

Extracellular Matrix (ECM) and Fibroblast Studies

Tripeptide-3 is a primary focus for research involving dermal cell cultures. The 200mg concentration allows for high-throughput screening to observe:

• Cellular Proliferation: Measuring the rate of fibroblast growth and migration.

• Collagen Synthesis Pathways: Investigating the upregulation of structural proteins in connective tissue models.

• Bio-availability Kinetics: Studying how the AHK sequence interacts with cellular receptors in the absence of metal ions.

Comparative Research and Precursor Modeling

By utilizing AHK (Tripeptide-3) 200mg, researchers can establish vital baseline data. This allows for comparative studies against copper-bound versions (AHK-Cu) to determine the specific contributions of the peptide sequence versus the metal complex. It is an essential reagent for labs focusing on:

• Tissue Engineering: Developing scaffolds for regenerative medicine.

• Gene Expression: Analyzing the modulation of mRNA levels associated with tissue repair.

• Protein Stability: Monitoring the half-life of small peptides in various biological media.